Tape-Arabidopsis Sandwich - a simpler Arabidopsis protoplast isolation method

Arabidopsis leaf plasma membrane proteome using a gel free method: Focus on receptor–like kinases

The hydrophobic proteins of plant plasma membrane still remain largely unknown.  For example in the Arabidopsis genome, receptor-like kinases (RLKs) are plasma membrane proteins, functioning as the primary receptors in the signaling of stress conditions, hormones and the presence of pathogens form a diverse family of over 610 genes. A limited number of these proteins have appeard in pr...

Isolation of the Arabidopsis GA4 locus.

Progeny from a transgenic Arabidopsis plant generated by the Agrobacterium root transformation procedure were found to segregate for a gibberellin (GA)-responsive semidwarf phenotype. Complementation analysis with genetically characterized GA-responsive mutants revealed that the transgenic plant has an insertional mutation (ga4-2) that is an allele of the ga4 locus. The semidwarf phenotype of g...

Rapid isolation of Arabidopsis thaliana developing embryos.

Identification and Characterization of LHCB1 Co-Suppressed Line in Arabidopsis

To explore the function of light-harvesting complex protein (LHCP) in Arabidopsis growth and development, the Leclere and Bartel seed collection was screened. In this collection randomly cloned cDNAs are expressed under the CaMV35S promoter. A pale green line has been identified and characterized in more details. Analysis of the inserted cDNA in the pale green line showed it encodes LHCB1 prote...

arabidopsis leaf plasma membrane proteome using a gel free method: focus on receptor–like kinases

the hydrophobic proteins of plant plasma membrane still remain largely unknown.  for example in the arabidopsis genome, receptor-like kinases (rlks) are plasma membrane proteins, functioning as the primary receptors in the signaling of stress conditions, hormones and the presence of pathogens form a diverse family of over 610 genes. a limited number of these proteins have appeard in protein pro...